To determine the fee for your specific project, the IDI-GEMS fee for a P3 cartridge ($6,780) serves as the baseline variable. Other cartridges--P1, P2--may be used and the fee for those would be applied, but in general, the P3 cartridge is the standard cartridge. The P3 cartridge allows IDI-GEMS to provide you with the highest value for your sequencing project by maximizing P3 cartridge space. This is accomplished by loading other customer projects onto the same P3 cartridge, which is a common practice in the industry.
Your specific project sequencing fee is calculated based on the number of reads per sample. A P3 cartridge provides for 2.4 billion reads. Therefore, if your specific sequencing project calls for 15 million reads, for example, the percentage of the P3 cartridge for your specific project is calculated as follows: 15,000,000 reads/2,400,000,000 reads = .00625.
The IDI-GEMS fee for a P3 cartridge is $6,780, so the sequencing fee in this example is: $6,780 x .00625 = $42.375 which is rounded to $42.38. In this example, let's assume there are 10 samples. The fee for sequencing would be: $42.38/sample x 10 samples = $423.80.
This will appear as a separate and distinct line item on your quote and subsequent invoice.
In addition to the P3 cartridge percentage, IDI-GEMS requires all samples to undergo Qubit based nucleic acid (DNA or RNA) quantification ($4 per sample) for quality assurance of inputs for library generation and pooling. Adding to the example above,
$4/sample x 10 samples = $40. Qubit based quantification (DNA or RNA) will appear
as a separate and distinct line item on your quote and subsequent invoice.
Also, a TapeStation quality analysis of the libraries will be performed. If you complete the libraries and provide these samples to IDI-GEMS, 100% of the libraries will undergo TapeStation quality analysis. If IDI-GEMS completes the library, then a determination
will be made by IDI-GEMS staff to conduct TapeStation quality analysis on all samples if the sample size is small or a percentage/number of samples if the number of samples is large.
Let's assume that IDI-GEMS is performing genomic sequencing on samples for which the library prep was completed by your lab. Using the same 10 samples as in the previous examples above and in the example the samples are DNA, then the IDI-GEMS staff
will conduct TapeStation analysis on all 10 samples. Therefore, the cost is $7.25/sample TapeStation analysis (DNA) will appear as a separate and distinct line item on your quote and subsequent invoice.
The fees (and associated calculations above) are the standard fees for IDI-GEMS genomic sequencing services. Additional fees (e.g., nucleic acid extraction and quantification, study design, bioinformatics) may also be part of your specific project. Fees for those services will be added and will appear as separate and distinct line items on your quote and subsequent invoice.
There may be additional fees (or quantities) that must be applied to your specific project. These will be included in the quote and subsequent invoice.
Finally, IDI-GEMS develops custom fees for unique projects that are not considered standard projects as determined by IDI-GEMS staff. In those situations, the fees are TBD as reflected on the IDI-GEMS service and fee list.